b phuket 3073 2013  (Native Antigen Inc)

Journal: Nature Communications

Article Title: Influenza vaccines promote humoral and cellular immune responses: a randomized, double-blind, phase 3 trial

doi: 10.1038/s41467-025-67102-y

Figure Lengend Snippet: Reciprocal titer of antibodies against several strains of influenza virus (A/H1N1, A/H3N3, B/Yamagata, and B/Victoria) was measured by hemagglutination inhibition assay (HAI) in subjects vaccinated with ( A ) Sinovac-QIV and with ( B ) Vaxigrip-Tetra TM . C The reciprocal titer of antibodies was compared between the two vaccines at 28 days post-immunization. The geometric mean titer of HAI antibodies against each strain was calculated by geometric mean and 95% CI. The data were transformed to Log 2 . The numbers in black correspond to pre- and post-immunization GMT, and the blue numbers correspond to the geometric mean increase, and the red numbers correspond to the geometric mean decrease. n = 131 (volunteers immunized with Vaxigrip-Tetra TM ) and n = 132 (volunteers immunized with Sinovac-QIV). A , B Statistical significance was assessed using a two-tailed paired t-test ( p < 0.0001 for all comparisons). C Data are presented as geometric mean values ± SEM. Two-tailed unpaired t-test (independent samples). p < 0.05 was considered statistically significant. Source data are provided in a file.

Article Snippet: HA protein derived from Influenza virus A/H3N2 (A/Darwin/9/2021, Sinobiological 40859-V08H) (2.5 μg/mL), Influenza B/Yamagata (B/PHUKET/3073/2013, Sinobiological 40498V08B) (2.5 μg/mL), Influenza B/Victoria (B/Austria/1359417/2021 (B/Victoria lineage)-like virus, Sinobiological 40862-V08B) (2.5 μg/mL) and Influenza A/H1N1 (A/Sydney/5/2021, Sinobiological 40944-V08B) (2.5 μg/mL) were used as specific stimuli.

Techniques: Virus, HI Assay, Vaccines, Transformation Assay, Two Tailed Test

Journal: Nature Communications

Article Title: Influenza vaccines promote humoral and cellular immune responses: a randomized, double-blind, phase 3 trial

doi: 10.1038/s41467-025-67102-y

Figure Lengend Snippet: PBMCs were stimulated for 48 h with 2.5 μg/mL of HA-derived proteins from A/H1N1, A/H3N2, B/Victoria, and B/Yamagata. The number of Spot Forming Cells (SFCs) secreting IFN-γ was quantified in PBMCs isolated from subjects vaccinated with ( A ) Sinovac-QIV and with ( B ) Vaxigrip-Tetra TM , before and after immunization. C The number of SFCs secreting IFN-γ was compared between the two vaccines at 28 days post-immunization. The data were normalized with the untreated control. Representative ELISPOT images for ( D ) Sinovac-QIV and E Vaxigrip-Tetra™ are shown, where red spots correspond to IFN-γ-secreting cells and blue spots correspond to IL-4-secreting cells: n = 27 (volunteers vaccinated with Vaxigrip-Tetra TM ) and n = 29 (volunteers vaccinated with Sinovac-QIV). Numbers above dots represent the mean. The numbers in blue represent fold increases, and the numbers in red represent fold reductions. A , B Statistical significance was assessed using a two-tailed paired t-test. C Data are presented as mean values ± SEM. Two-tailed unpaired t-test (independent samples). p < 0.05 was considered statistically significant. Source data are provided in a file.

Article Snippet: HA protein derived from Influenza virus A/H3N2 (A/Darwin/9/2021, Sinobiological 40859-V08H) (2.5 μg/mL), Influenza B/Yamagata (B/PHUKET/3073/2013, Sinobiological 40498V08B) (2.5 μg/mL), Influenza B/Victoria (B/Austria/1359417/2021 (B/Victoria lineage)-like virus, Sinobiological 40862-V08B) (2.5 μg/mL) and Influenza A/H1N1 (A/Sydney/5/2021, Sinobiological 40944-V08B) (2.5 μg/mL) were used as specific stimuli.

Techniques: Derivative Assay, Isolation, Vaccines, Control, Enzyme-linked Immunospot, Two Tailed Test

Journal: Nature Communications

Article Title: Influenza vaccines promote humoral and cellular immune responses: a randomized, double-blind, phase 3 trial

doi: 10.1038/s41467-025-67102-y

Figure Lengend Snippet: A Clustering analyses with the Phenograph algorithm (Euclidean distance, 350 neighbors) grouped CD4 + AIM + T cells in 29 clusters. B Relative expression levels of CD137, ICOS, CD45RA, Lag3, PD1, CCR7, CD69, CD38, OX40, and CXCR5 for each cluster were quantified and represented in a heatmap. Phenotype profile and changes in frequency of CD4 + AIM + T cell clusters following vaccination with Sinovac-QIV and Vaxigrip-Tetra TM in response to ( C ) A/H1N1, ( D ) A/H3N2, ( E ) B/Vitoria, and ( F ) B/Yamagata. n = 27 (volunteers immunized with Sinovac-QIV) and n = 23 (volunteers immunized with Vaxigrip-Tetra TM ). Numbers above dots represent the mean. For each strain, a two-way mixed-effects ANOVA was performed, followed by Holm–Šídák’s multiple comparisons test. Paired comparisons were conducted within each vaccine group (day 0 vs. day 28), and unpaired comparisons were performed between vaccine groups at day 28. p < 0.05 was considered statistically significant. Source data are provided in a Source Data file.

Article Snippet: HA protein derived from Influenza virus A/H3N2 (A/Darwin/9/2021, Sinobiological 40859-V08H) (2.5 μg/mL), Influenza B/Yamagata (B/PHUKET/3073/2013, Sinobiological 40498V08B) (2.5 μg/mL), Influenza B/Victoria (B/Austria/1359417/2021 (B/Victoria lineage)-like virus, Sinobiological 40862-V08B) (2.5 μg/mL) and Influenza A/H1N1 (A/Sydney/5/2021, Sinobiological 40944-V08B) (2.5 μg/mL) were used as specific stimuli.

Techniques: Expressing

Journal: Nature Communications

Article Title: Influenza vaccines promote humoral and cellular immune responses: a randomized, double-blind, phase 3 trial

doi: 10.1038/s41467-025-67102-y

Figure Lengend Snippet: A Clustering analyses with the Phenograph algorithm (Euclidean distance, 350 neighbors), and grouped CD8 + AIM + T cells in 21 clusters. B Relative expression levels of CD137, ICOS, CD45RA, Lag3, PD1, CCR7, CD69, CD38, OX40, and CXCR5 for each cluster were quantified and represented in a heatmap. Phenotype profile and changes in frequency of CD4 + AIM + T cell clusters following vaccination with Sinovac-QIV and Vaxigrip-Tetra TM in response to ( C ) A/H1N1, ( D ) A/H3N2, ( E ) B/Vitoria, and ( F ) B/Yamagata. Numbers above dots represent the mean. n = 26 (volunteers immunized with Sinovac-QIV) and n = 19 (volunteers immunized with Vaxigrip-Tetra TM ). Numbers above dots represent the mean. For each strain, a two-way mixed-effects ANOVA was performed, followed by Holm–Šídák’s multiple comparisons test. Paired comparisons were conducted within each vaccine group (day 0 vs. day 28), and unpaired comparisons were performed between vaccine groups at day 28. p < 0.05 was considered statistically significant. Source data are provided in a Source Data file.

Article Snippet: HA protein derived from Influenza virus A/H3N2 (A/Darwin/9/2021, Sinobiological 40859-V08H) (2.5 μg/mL), Influenza B/Yamagata (B/PHUKET/3073/2013, Sinobiological 40498V08B) (2.5 μg/mL), Influenza B/Victoria (B/Austria/1359417/2021 (B/Victoria lineage)-like virus, Sinobiological 40862-V08B) (2.5 μg/mL) and Influenza A/H1N1 (A/Sydney/5/2021, Sinobiological 40944-V08B) (2.5 μg/mL) were used as specific stimuli.

Techniques: Expressing